Fig. 2

LM activates inflammasomes and stimulates pro-inflammatory cytokine production of mouse B6 macrophages (a) IL-1β concentrations in the media of B6 macrophages infected by LM at different MOI (5, 10, 20 and 40). b Western blot detection of pro-caspase-1 and activated caspase-1 in B6 macrophages following different treatments. c IL-1β concentrations in the culture media of B6 macrophages infected by LM (MOI = 20) and treated with different stimuli, including LM/zYV (zYVAD-FMK) (10 μM) as a negative control and LPS/ATP (0.5 μg/mL/5 mM) as a positive control for inflammasome activation. d Pro-IL-1β and TNFα detection in macrophages by RT-PCR. e IL-1β concentrations in the media of B6 macrophages infected with wild-type LM and Δhly LM (MOI = 20). f Western blot detection of pro-caspase-1 and its active form in infected macrophages infected with wild-type LM and Δhly LM (MOI = 20). The data in (a), (c) and (e) are shown as means ± standard deviation, and representative of three independent experiments. * p < 0.05; ** p < 0.01. NS: no significance