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Fig. 1 | BMC Veterinary Research

Fig. 1

From: NLRP3 inflammasome activation contributes to Listeria monocytogenes-induced animal pregnancy failure

Fig. 1

Effects of LM infection on embryo survival, caspase-1 expression/activation and IL-1β production in pregnant mice (a) Embryo survival rates of pregnant mice (n = 8) following infection with LM or Δhly LM at different doses (5 × 104, 5 × 105 and 5 × 106 CFU LM/ mouse in 0.2 mL PBS). b Pro-caspase-1 (P45) and activated caspase-1 (P10) levels in placental tissues detected by Western blot. c IL-1β levels measured by ELISA in the sera of pregnant mice infected with LM or Δhly LM at the same doses as above. d IL-1β levels (including pro-IL-1β and mature IL-1β) detected by ELISA in the placenta of LM- and Δhly LM-infected mice. e NLRP3 and ASC expressions in placenta of LM- and Δhly LM-infected mice analyzed by Western blot. f The effects of IL-1β antibody on embryo survival rates of LM-infected pregnant mice. The pregnancy mice were treated with different amount of mouse IL-1β antibody (0, 2, 4, 6, 8, 10 μg/d) for 3 days, and infected with LM on the 2nd day of antibody treatment. The data are presented as means ± standard deviation. * p < 0.05; ** p < 0.01. NS: no significance

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