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Fig. 5 | BMC Veterinary Research

Fig. 5

From: Differential intracellular calcium influx, nitric oxide production, ICAM-1 and IL8 expression in primary bovine endothelial cells exposed to nonesterified fatty acids

Fig. 5

NEFA effects on ICAM-1 and IL-8 expression in BUVECs. Cells were treated with 0.1 % ethanol, 300 μM myristic acid (MA), 0.1 % DMSO, 300 μM oleic acid (OA), or 300 μM linoleic acid (LA) for 3 h and their RNAs were then isolated. Some cells were treated with 10 μM GW1100 or 4 μM U73122 (U-73) for 30 min before the LA treatment. The expression of ICAM-1 and IL-8 was analyzed with real-time PCR using specific primers. Relative abundance = 2–ΔCt, where ΔCt = cycle treshold ICAM-1 or Ct IL-8 minus cycle treshold SDHA. The data are expressed as the means ± SEM of three experiments. *p < 0.05

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BMC Veterinary Research

ISSN: 1746-6148

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