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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Differential intracellular calcium influx, nitric oxide production, ICAM-1 and IL8 expression in primary bovine endothelial cells exposed to nonesterified fatty acids

Fig. 2

Signaling routes of the calcium responses caused by MA and LA in BUVECs. a and d Dose–response of calcium signaling induced by myristic acid (MA) or linoleic acid (LA). The area under the curve (AUC) calculated for the first 100 s of the Fura-2/AM time course. b and e Average time courses for the Fura-2 ratio in BUVECs exposed to 100 μM MA in ethanol or 30 μM LA in 1 % DMSO. Graph inset: whole time course of the Fura-2/AM ratio after exposure to 100 μM MA before the addition of 132 μM digitonin (dig) (representative assay). c and f Calcium response in cells triggered by 1 % ethanol or 1 % DMSO, 100 μM MA or 30 μM LA alone or after preincubation with 100 μM 2-APB, 10 μM GW1100, 4 μM U73122 (U7), or 20 μM nifedipine (NIFE) for 15 min. Cells were incubated for 16 h with 100 ng/ml pertussis toxin (PTX) before MA or LA supplementation. The data are expressed as the means ± SEM of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.005

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BMC Veterinary Research

ISSN: 1746-6148

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