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Table 2 Summary of the scrapie transmission results in sheep transfused with PBMC, monocytes or T lymphocytes from preclinical sheep with classical ovine scrapie infection

From: Classical scrapie prions are associated with peripheral blood monocytes and T-lymphocytes from naturally infected sheep

Group Donor ID Cell type Cell numbera Lamb PRNP   PrPSc detection by IHCb sPMCA
    Antemortem Postmortem Antemortem
Recipients Controls Animal ID Rectal tissues Rectal tissues Other lymphoid tissuesc Brains PBMC
4 6 12 18 21 10 25 10 25 10 25 15 17#
1 4454 PBMC 1.3 × 108 VRQ/VRQ   4599 (−) (−) NA NA NA (+) NA (+) NA (−) NA NA NA
     VRQ/VRQ   4601 (−) (+) NA NA NA (+) NA (+) NA (−) NA NA NA
  4438   4.5 × 108 VRQ/VRQ   4598 (−) (−) NA NA NA (−) NA (+) NA (−) NA NA NA
     VRQ/VRQ   4600 (−) (−) NA NA NA (+) NA (+) NA (−) NA NA NA
    NA   VRQ/VRQ 4602 (−) (−) NA NA NA (−) NA (−) (−) (−) NA NA NA
      VRQ/VRQ 4603 (−) (−) NA NA NA (−) NA (−) (−) (−) NA NA NA
2 4454 Monocytes 2.2 × 106 VRQ/VRQ   4594 (−) ND (−) (−) (−) NA (−) NA (−) NA (−) (−) ND
     VRQ/VRQ   4595 (−) ND (+) (+) (+) NA (+) NA (+) NA (+) (P) ND
  4438   4.5 × 106 ARQ/VRQ   4546 (−) ND (−) (−) (−) NA (−) NA (−) NA (−) (−) ND
     VRQ/VRQ   4593 (−) ND (−) (−) (−) NA (−) NA (−) NA (−) (−) ND
     VRQ/VRQ   4597 (−) ND (+) (+) (+) NA (+) NA (+) NA (+) (P) ND
    NA   VRQ/VRQ 4596 (−) ND (−) (−) ND NA (−) NA (−) NA (−) (−) ND
3 4454 T lymphocytes 5.9 × 106 VRQ/VRQ   4539 (−) ND (−) (−) (−) NA (−) NA (−) NA (−) ND (−)
     VRQ/VRQ   4590 (−) ND (−) (+) (+) NA (+) NA (+) NA (+) ND (P)
  4438   9.9 × 106 VRQ/VRQ   4588 (−) ND (−) (−) (−) NA (−) NA (−) NA (−) ND (−)
     VRQ/VRQ   4589 (−) ND (−) (−) (−) NA (−) NA (−) NA (−) ND (−)
    NA   VRQ/VRQ 4591 (−) ND (−) (−) ND NA (−) NA (−) NA (−) ND (−)
      VRQ/VRQ 4592 (−) ND (−) (−) ND NA (−) NA (−) NA (−) ND (−)
  1. Abbreviations: PRNP prion genotype, PBMC peripheral blood mononuclear cells, NA not applicable, ND Not done, sPMCA Serial protein misfolding cyclic amplification, P positive for prion protein misfolding activity; #months post-inoculation; (+) = PrPSc immunolabeling was detected; (−) = PrPSc immunolabeling was not detected; aindicate the total number of PBMC, monocytes or T lymphocytes isolated from a 50 mL whole blood sample volume; bPrPSc immunolabeling in the tissues were detected using a mixture of prion mAbs F99/97.6.1 and F89/160.1.5 by immunohistochemistry; cOther lymphoid tissues include alimentary tract-associated lymphoid tissues (tonsils, retropharyngeal, mesenteric such as ruminal, abomasal, duodenal, jejunal, ileocecal, distal ileum, ileocecal junction lymph nodes, ileum, and spleens) and peripheral lymph nodes (prescapular, prefemoral, and popliteal)