Table 2 List of primary antibodies used for immunohistochemistry and western blotting analysis
From: Extracellular matrix remodeling in equine sarcoid: an immunohistochemical and molecular study
Antibody | Manufacturer | Clone | Specificity | Host species | Antigen retrieval | IHC Dilution | WB Dilution |
|---|---|---|---|---|---|---|---|
VIMENTIN | Dako cytomation | V-9 | man, cow, dog, hamster, horse, rabbit, rat | mouse | No antigen retrieval | 1:50 | - |
SMOOTH MUSCLE ACTIN | Dako cytomation | 1-a-4 | chicken, cow, rat | mouse | Citrate, ph 6.0, 30 min, steamer | 1:50 | 1:5000 |
TYPE I COLLAGEN | Ab-cam | Col-1 | rat, rabbit, cow, human, pig, deer | mouse | Citrate, ph 6.0, 30 min, steamer | 1:100 | 1:5000 |
TYPE III COLLAGEN | Millipore | Ie7-d7 | rabbit, human | mouse | Citrate, ph 6.0, 30 min, steamer | 1:100 | 1:5000 |
MMP-2 | Thermo-scientific | Ab-7 | human, mouse, rat, cow | rabbit | Citrate, ph 6.0, 30 min, steamer | 1:200 | 1:500 |
MMP-9 | Millipore | 56-2a4 | human, rat, rabbit, guinea pig | mouse | Citrate, ph 6.0, 30 min, steamer | 1:200 | 1:1000 |
MT1-MMP (MMP-14) | Millipore | 113-5b7 | human | mouse | Citrate, ph 6.0, 30 min, steamer | 1:200 | 1:500 |
TIMP-2 | Millipore | 2TMP05 | bovine, guinea pig, human, mouse, rat, rabbit | mouse | Citrate, ph 6.0, 30 min, steamer | 1:200 | 1:200 |