Table 1 Sequences of primers used for the PCRs
Primers | Sequences (5′-3′) | Underlined restriction site |
|---|---|---|
A1 | TTATCCATGGGCCACCACCACCACCACCACACTACTCCTCCCGCCAT ACAAT | NcoI |
A2 | GCCGAATTCGCCAAAGCTGATTCTT | EcoRI |
B1 | ATGAATTCACTACTCCTCCCGCCATAC | EcoRI |
B2 | TTGGAGCTCGCCAAAGCTGATTCCT | SacI |
C1 | ATAGAGCTCACTACTCCTCCCGCCATACAAT | SacI |
C2 | CGAAGCTTATTAGCCAAAGCTGATTCCTTTTGTT | Hind III |