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Table 1 Sequences of primers used for the PCRs

From: Development and application of an indirect enzyme-linked immunosorbent assay using recombinant truncated Cap protein for the diagnosis of porcine circovirus-like virus P1

Primers Sequences (5′-3′) Underlined restriction site
A1 TTATCCATGGGCCACCACCACCACCACCACACTACTCCTCCCGCCAT ACAAT NcoI
A2 GCCGAATTCGCCAAAGCTGATTCTT EcoRI
B1 ATGAATTCACTACTCCTCCCGCCATAC EcoRI
B2 TTGGAGCTCGCCAAAGCTGATTCCT SacI
C1 ATAGAGCTCACTACTCCTCCCGCCATACAAT SacI
C2 CGAAGCTTATTAGCCAAAGCTGATTCCTTTTGTT Hind III