Fig. 2

Identification of the rctCap protein with SDS-PAGE and western blotting. M: protein molecular weight marker; 1: IPTG-induced cell lysate; 2: rctCap protein purified with a Ni2+-NTA affinity chromatography column; 3: the purified rctCap protein reacted strongly with rabbit anti-P1 polyclonal antibody detected with HRP- conjugated goat anti-rabbit antibody as the secondary antibody