Skip to main content

Table 3 Primer and probe sequences used for positive control and qPCR assays

From: Biomarkers of selenium status in dogs

Gene

Forward primer

Reverse primer

Probe

Product size

GPx1

GTTCGGGCATCAGGAAAAC

TTCACCTCGCACTTCTCAAA

AAGTTGGGCTCGAACCCGCC

114

SelH

GGAGCTTTGGACTGGGATT

CTTCTGCCCAAACACCCTAC

CTTGAGTTTGCGTGGGGGCC

113

SepW1

GGCTACAAGTCCAAGTACCTTCAG

CCTCTCTTCTTGGAGTGAACCA

AAAGAAGCCGGTGGCCTGGG

148

TrxRd1

GTAGCAATCCAGGCAGGAAG

CAGGCACCATATTCCAAAGG

TGGCTCAGAGGCTTTATGCTGGC

116

TrxRd2

TTTATGCCATCGGAGACGTAG

CCATTATAGCCGTGGGTGTC

AGAGGGGCGGCCCGAGCT

60

DIO1

GAGGCTCTGGGTCCTCTTG

CCACGATGTGTTGCTTGACT

AGGTGGCCGTGGGCAAAGTG

95

SelK

AGGCTATGGAAACTCCTCTGATT

TTAATTCGACCCATTCTTCTGG

TGATGGAAGAGGGCCACCAGG

84

SelT

CGCTGCTCAAGTTCCAGATT

CCGCATGTACTCCTCAAACA

TGTGTTTCCTGAGGTTATAGGCGGG

65

Sep15

GGTCCTTCAAGCGGTGTCT

CAAGTTGCTGGAGAAGCCTAA

CGGGGCAGAGTTTTCATCAGAAGC

79

TNF-Ī±

CATGTGCTCCTCACCCACAC

AGGGCTCTTGATGGCAGAGA

CGCTTCGCCGTCTCCTACCA

84

NFĪŗB1

TGAGGATGGGATCTGCACT

CTCTGTCATTCGTGCTTCCA

TGGTCGGCTTTGCAAACCTGGG

124

B2M

ACGGAAAGGAGATGAAAGCA

CCTGCTCATTGGGAGTGAA

AGACCTGTCTTTCAGCAAGGACTGGACC

99

GAPDH

TCAACGGATTTGGCCGTATTGG

TGAAGGGGTCATTGATGGCG

CAGGGCTGCTTTTAACTCTGGCAAAGTGGA

90

HPRT1

CACTGGGAAAACAATGCAGA

ACAAAGTCAGGTTTATAGCCAACA

TGCTGGTGAAAAGGACCCCTCG

123

  1. qPCR quantitative polymerase chain reaction, GPx1 glutathione peroxidase 1, SelH selenoprotein H, SepW1 selenoprotein W, TrxRd1 thioredoxin reductase 1, TrxRd2 thioredoxin reductase 2, DIO1 iodothyronine deiodinase 1, SelK selenoprotein K, SelT selenoprotein T, Sep15 15Ā kDa selenoprotein, TNF-Ī± tumor necrosis factor alpha, NFĪŗB1 nuclear factor kappa-light-chain-enhancer of activated B cells, B2M beta-2-microglobulin, GAPDH glyceraldehyde 3-phosphate dehydrogenase, HPRT1 hypoxanthine phosphoribosyltransferase 1