Biomarkers of selenium status in dogs

BMC Veterinary Research

Table 2 Analysed^a chemical composition of two semi-purified diets with an adequate or low selenium concentration

Component (g/MJ, except where specified)	Adequate Se diet	Low Se diet
Dry matter (g/100 g as is)	92.8	92.7
Crude protein	14.9	14.9
Crude fat	5.8	5.8
Crude fibre	1.6	1.5
Nitrogen free extract^b	35.5	35.4
Starch	13.6	13.1
Crude ash	2.5	2.4
Metabolisable energy (MJ/kg DM)^c	16.6	16.7
Amino acids
Arginine	0.38	0.40
Histidine	0.21	0.21
Isoleucine	0.38	0.37
Leucine	0.68	0.68
Lysine	0.36	0.38
Methionine	0.32	0.36
Cysteine	0.36	0.36
Phenylalanine	0.45	0.47
Tyrosine	0.23	0.23
Threonine	0.47	0.47
Tryptophan	0.14	0.14
Valine	0.51	0.52
Fatty acids
Linoleic acid	1.35	0.96
α-Linolenic acid (mg/MJ ME)	91	113
Arachidonic acid (mg/MJ ME)	22.6	9.3
EPA & DHA (mg/MJ ME)	6.9	6.5
Minerals (mg/MJ ME)
Potassium	416	414
Calcium	383	388
Phosphorus	299	304
Chloride	123	110
Sodium	97	91
Magnesium	58.4	58.2
Zinc	6.5	6.5
Iron	3.4	3.9
Copper	0.48	0.68
Manganese	0.73	0.57
Iodine	0.08	0.08
Selenium (μg/MJ ME)	46.1	6.5
Vitamins (mg/MJ ME)
Choline	148	146
Biotin	46.1	39.3
Niacin	5.5	4.9
Pantothenic acid	1.8	2.0
d-α-tocopherol	1.8	1.8
Riboflavin	0.45	0.44
Thiamin	0.24	0.27
Pyridoxine	0.14	0.22
Retinol	0.16	0.16
Menadion (μg/MJ ME)	71.4	90.6
Folic acid (μg/MJ ME)	53.2	45.2
Cyanocobalamin (μg/MJ ME)	35.6	37.5
Cholecalciferol (μg/MJ ME)	1.13	0.94

MJ megajoule, ME metabolisable energy, EPA Eicosapentaenoic acid, DHA docosahexaenoic acid
^aAll analyses were performed by Eurofins Food Testing, Wolverhampton, UK
^bCalculated by substracting the amount (as g/100 g as is) of crude fat, protein, ash and fibre from the percentage dry matter and dividing this by the metabolisable energy concentration (MJ/kg as is)
^cCalculated using predictive equations for ME (NRC, 2006)
Both dog groups received the adequate Se diet in the 8-week pre-feeding period. Dog group A continued to receive the adequate Se diet in the 8-week experimental period and dog group B switched to the low Se diet

ISSN: 1746-6148