Assessment of the main plasma parameters included in a metabolic profile of dairy cow based on Fourier Transform mid-infrared spectroscopy: preliminary results

Table 1 Methodologies used to measure the plasma parameters with reference methods

Parameter	Methodology	Wavelength (nm)	CV^a
Glucose	Endpoint	510	1.50
Total cholesterol	Endpoint	510	2.10
NEFA^b	Endpoint	546	1.50
BHBA^c	Endpoint	340	5.25
Urea	Endpoint	340	1.20
Creatinine	Endpoint	510	5.40
Ca	Endpoint	570	1.40
Inorganig P	Endpoint	340	2.00
Mg	Rate	340	1.40
Na	ISE device^g		0.90
K	ISE device^g		1.30
Cl	ISE device_g		1.50
Zn	Endpoint	546
Ceruloplasmin	Endpoint	546	3.48
Total protein	Endpoint	546	1.20
Albumin	Endpoint	600	1.80
Total bilirubin	Endpoint	546	6.70
Haptoglobin	Endpoint	450	13.54
AST^d	Rate	340	2.10
GGT^e	Rate	405	3.72
AP^f	Rate	405	1.70

^aCalculared on the results obtained between runs according to the National Committee for Clinical Laboratory Standards (Document EP3-T: Guidelines for Manufacturers for Establishing
Performance Claims for Clinical Chemistry Methods, Replication Experiment
Evaluation”, Villanova, PA, 1982.)
^bNon esterified fatty acids;
^cβ-OH-butyric acid;
^dAspartate amino transferase
^eγ-glutamyl transferase
^fAlkaline phosphatase
^gIon selective electrodes

ISSN: 1746-6148