Effect of Cyclooxygenase(COX)-1 and COX-2 inhibition on furosemide-induced renal responses and isoform immunolocalization in the healthy cat kidney

Table 1 COX-1 and COX-2 scores in the kidney of normal young and old cats

COX-1 immunostaining	Young (n = 10)	Old (n = 11)
Thick limb	1.37 [1.01–1.66]	1.75 [1.43–1.81]
Collecting duct	1.32 [0.98–1.82]	1.41 [1.25–1.66]
Distal tubule	1.10 [0.84–1.35]	1.14 [1.00–1.43]
Thin limb	0.97 [0.58–1.20]	1.42 [0.82–1.55]
Macula densa	0.75 [0.55–0.81]	0.82 [0.80–1.13]
Proximal tubule	0.56 [0.44–0.71]	0.74 [0.36–0.91]
Glomerulus	0.48 [0.09–1.01]	0.70 [0.15–1.00]
Arteriole	0.50 [0.28–0.80]	0.60 [0.40–0.70]
Medullary interstitial cell	0.00 [0.0–0.25]	0.00 [0.00–0.00]
COX-2 immunostaining	Young (n = 10)	Old (n = 11)
Macula densa	1.29 [0.71–2.18]	1.32 [1.00–2.03]
Thin limb	1.16 [0.65–1.64]	1.10 [0.75–1.64]
Thick limb	1.10 [0.77–1.19]	0.93 [0.75–1.16]
Proximal tubule	0.73 [0.41–0.85]	1.19 [0.72–1.30]
Medullary interstitial cell	1.00 [0.00–1.00]	1.00 [0.00–1.00]
Collecting duct	0.81 [0.38–1.12]	0.90 [0.60–1.01]
Distal tubule	0.45 [0.27–1.13]	0.86 [0.42–1.09]
Glomerulus	0.75 [0.31–1.41]	0.80 [0.65–2.00]
Arteriole	0.15 [0.00–2.23]	0.10 [0.00–0.30]

For each cat, an average intensity score for each structure was calculated as described in the methods section (range 0 to 3). This single value has been used to calculate the median [interquartile range] value presented in the table for each group of cats. There was no difference in expression between young and old cats for either isoenzyme (P > 0.05)

ISSN: 1746-6148