Fig. 4

Comparison of the anticoccidial activities of M. guilliermondii 01 whole culture, culture supernatant, ethyl acetate extract and intracellular proteins on E. tenella oocyst After 48 h, the most severe damage of the oocyst wall was observed in bioassays with M. guilliermondii 01 culture and ethyl acetate extract showing 22.16 and 18.90 % of initial integrity, respectively. M. guilliermondii 01 intracellular proteins and supernatant culture also showed substantial anticoccidial activity, with 45 and 42.40 % of initial integrity after 48 h, respectively. This result suggests that anticoccidial activity is related to a excreted product and also an intracellular compound. Significant differences among treatments and control at 24 hare shown with an asterisk and significant differences among treatments and control at 48 h are shown with double asterisk (significance threshold, P < 0.05)