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Fig. 3 | BMC Veterinary Research

Fig. 3

From: Porcine Mx1 fused to HIV Tat protein transduction domain (PTD) inhibits classical swine fever virus infection in vitro and in vivo

Fig. 3

Antiviral activity in ST (a) or 3D4/21 (b) cells in response to three different PTD-poMx1 treatments. Cells were treated with 80 μg/ml PTD-poMx1 and 0.01 MOI CSFV as follows: (i) cells pretreated with PTD-poMx1, followed by CSFV infection; (ii) cells co-inoculated with PTD-poMx1 and CSFV; (iii) cells infected with CSFV, then treated with PTD-poMx1. At 48 hpi, virus was released from cells by freezing and thawing three times, and qRT-PCR was performed to evaluate viral RNA load in vitro. Experiments were performed in triplicate and data are shown as mean ± S.D. Statistical significance is indicated as *(P < 0.05)

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