Table 2 Results of all methods applied to the samples taken from dogs and ticks that showed growth of a Leishmania culture from the material obtained from the ticks, Federal District, Brazil, 2011
Dogs | Ticks | Cultures | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Dog | ELISA | IFI | DPP | PCR Canine blood (kDNA) | PCR Canine blood (hsp70) | Collected ticks | Tick sex (n) | PCR (kDNA) Pool SG | PCR (kDNA) Pool Int. | Culture (NNN) | PCR (kDNA) Culture | PCR (hsp70) Culture |
I | Reagent | 1/80 | Positive | Positive | Negative | 6 | Male (1) | Positivo | Negative | Negative | ND | ND |
Female (5) | Positive | Positive | Positive | Positive | Positive | |||||||
II | Reagent | 1/80 | Positive | Positive | Negative | 5 | Male (5) | Positive | Positive | Positive | Negative | Negative |
III | Reagent | 1/80 | Positive | Positive | Positive | 8 | Male (5) | Positive | Positive | Positive | Positive | Positive |
Female (3) | Positive | Positive | Positive | Positive | Positive | |||||||
IV | Reagente | 1/40 | Positive | Positive | Positive | 2 | Male (1) | Negative | Negative | Negative | ND | ND |
Female (1) | Positive | Positive | Positive | Positive | Positive | |||||||