Fig. 2

Analysis of binding specificity of anti-STIV ssDNA aptamers. a EMSA of selected aptamers incubated with STIV (lane 1, 7.5 μg aptamer only; lanes 2–4, STIV incubated with 7.5, 15 or 30 μg of each aptamer, respectively; lane 5, 30 μg of aptamer only; and lane 6, STIV only). b EMSA of selected aptamers incubated with SGIV. Note that no bands were visible in lanes 5–9, which proved the specificity of selected aptamers only recognizing STIV. c, d Fluorescent localization of STIV-aptamer binding in virus particles stained with Hoechst 33,342. c Imaging of FITC-aptamer-labeled STIV virus particles by fluorescence microscopy. d Controls of FITC-aptamers not binding to SGIV, and initial library not binding to STIV. (Bars = 50 μm)