Fig. 2

COBRA and MSP for Osteosarcoma, Primary Lung Cancer, and Normal Control Lung. PCR products were run on a 1.5 % agarose gel. Lad = 100 bp ladder. Samples labeled O-X are osteosarcoma, P-X are primary lung cancer, and C-X are control lung. X represents the patient number. Sssi is normal canine spleen DNA methylated in-vitro and Spl is normal canine spleen DNA. Sample C-7 did not amplify by MSP