Endocrine control of canine mammary neoplasms: serum reproductive hormone levels and tissue expression of steroid hormone, prolactin and growth hormone receptors

Table 1 List of primers and TaqMan probes used for the semi-quantitative real-time (TaqMan) PCR

Primer	Accession number	Primer sequence	Product length (bp)
ERα (ESR1)	XM533454	Forward: 5’-CCC ATG GAG GAG ACA AAC CA-3’,	93
		Reverse: 5’-CCC TGC CTC CTC GGT GAT ATA-3’
		TaqMan probe: 5’-CAC GGG CCC AAC TTC ATC ACA TTC-3’
ERβ (ESR2)	XM861041	Forward: 5’-CCC AGC ACG CCC TTC A-3’	78
		Reverse: 5’-AAT CAT ATG CAC GAG TTC CTT GTC-3’
		TaqMan probe: 5’-CCT CCA TGA TGA TGT CCC TGA CC-3’
GAPDH	AB028142	Forward: 5’-GCT GCC AAA TAT GAC GAC ATC-3’	75
		Reverse: 5’-GTA GCC CAG GAT GCC TTT GAG-3’
		TaqMan probe: 5’-TCC CTC CGA TGC CTG CTT CAC TAC CTT-3’
PGR	NM_001003074	Forward: 5’-CGA GTC ATT ACC TCA GAA GAT TTG TTT-3’	113
		Reverse: 5’-CTT CCA TTG CCC TTT TAA AGA AGA-3’
		TaqMan probe: 5’-AAG CAT CAG GCT GTC ATT ATG GTG TCC TAA CTT-3’
PRL	NM_00108275	Forward: 5’-CAA GCC CAA CAG ATC CAC CAT-3’	104
		Reverse: 5’-ATC CCC CGC ACT TCT GTG A-3’
		TaqMan probe: 5’-CTG AGG GTG CTG CGC TCC TGG-3’
PRLR	HQ267784	Forward: 5’-GGA TCT TTG TTG CCG TTC TTT -3’	92
		Reverse: 5’-AAG GAT GCA GGT CAC CAT GCT AT-3’
		TaqMan probe: 5’-ATT ATG GTC GTA GCA GTG GCT TTG AAA GGC-3’
18SrRNA	FJ797658	Forward: 5’-GTC GCT CGC TCC TCT CCT ACT-3’	125
		Reverse: 5’-GGC TGA CCG CCT TGG TTT-3’
		TaqMan probe: 5’-ACA TGC CGA CGG GCG CTG AC-3’

ISSN: 1746-6148