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Table 1 List of primers and TaqMan probes used for the semi-quantitative real-time (TaqMan) PCR

From: Endocrine control of canine mammary neoplasms: serum reproductive hormone levels and tissue expression of steroid hormone, prolactin and growth hormone receptors

Primer Accession number Primer sequence Product length (bp)
ERα (ESR1) XM533454 Forward: 5’-CCC ATG GAG GAG ACA AAC CA-3’, 93
Reverse: 5’-CCC TGC CTC CTC GGT GAT ATA-3’
TaqMan probe: 5’-CAC GGG CCC AAC TTC ATC ACA TTC-3’
ERβ (ESR2) XM861041 Forward: 5’-CCC AGC ACG CCC TTC A-3’ 78
Reverse: 5’-AAT CAT ATG CAC GAG TTC CTT GTC-3’
TaqMan probe: 5’-CCT CCA TGA TGA TGT CCC TGA CC-3’
GAPDH AB028142 Forward: 5’-GCT GCC AAA TAT GAC GAC ATC-3’ 75
Reverse: 5’-GTA GCC CAG GAT GCC TTT GAG-3’
TaqMan probe: 5’-TCC CTC CGA TGC CTG CTT CAC TAC CTT-3’
PGR NM_001003074 Forward: 5’-CGA GTC ATT ACC TCA GAA GAT TTG TTT-3’ 113
Reverse: 5’-CTT CCA TTG CCC TTT TAA AGA AGA-3’
TaqMan probe: 5’-AAG CAT CAG GCT GTC ATT ATG GTG TCC TAA CTT-3’
PRL NM_00108275 Forward: 5’-CAA GCC CAA CAG ATC CAC CAT-3’ 104
Reverse: 5’-ATC CCC CGC ACT TCT GTG A-3’
TaqMan probe: 5’-CTG AGG GTG CTG CGC TCC TGG-3’
PRLR HQ267784 Forward: 5’-GGA TCT TTG TTG CCG TTC TTT -3’ 92
Reverse: 5’-AAG GAT GCA GGT CAC CAT GCT AT-3’
TaqMan probe: 5’-ATT ATG GTC GTA GCA GTG GCT TTG AAA GGC-3’
18SrRNA FJ797658 Forward: 5’-GTC GCT CGC TCC TCT CCT ACT-3’ 125
Reverse: 5’-GGC TGA CCG CCT TGG TTT-3’
TaqMan probe: 5’-ACA TGC CGA CGG GCG CTG AC-3’