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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Activity of the DNA minor groove cross-linking agent SG2000 (SJG-136) against canine tumours

Fig. 2

SG2000-induced cell cycle alterations in four canine melanoma cell lines following a 1 h treatment with equitoxic (GI50) doses of SG2000 and 72 h of post-incubation. The doses used were LMeC 24nM, KMeC 4.7 nM, CMeC-1 1 μM and CMeC-2 300 nM. Values represent the percentage of cells in the G1/G0 (black), S (white), and G2/M (grey) phases of the cell cycle according to DNA content per cell, assessed by flow cytometry. The percentage of cells in each phase was calculated from a minimum of 10,000 events and the values shown represent the mean from three independent experiments ± SEM

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