Fig. 4

Expression of inflammatory and cell cycle progression markers by flow cytometry on cells derived from the synovial fluid and membrane. The TNF-r, MCP-1 and CD1a were significantly reduced in the synovial membrane cells compared to the synovial fluid cells. There was no difference in relation to the expression of the other markers (Cox-2 and CD11) between the two cultures and all markers showed significant expression. In relation to cell cycle progression markers, statistical difference between the cultures was observed only in relation to the expression of HSP47, which was increased in the synovial fluid cultures. The expression of other markers (Caspase-3, p21, Ki67, ciclin-D1, and p53) was similar between both cultures