Table 1 Primary antibodies used for immunohistochemistry
Antibody | Clone | Source | Dilution | Staining pattern | Labelling |
|---|---|---|---|---|---|
Monoclonal mouse anti-human estrogen receptor (ER) | ER-12 | Cell Marque®, USA | 1:40 | Nuclear | Novolink® Polymer Detection System, (RE7280-K) Leica Biosystems®, UK |
Monoclonal mouse anti-human progesterone receptor (PR) | 1A6 | Novocastra®, UK | 1:30 | ||
Monoclonal Mouse anti-human Ki-67 Antigen | MIB-1 | Dako®, Denmark | 1:50 | ||
Clonal rabbit anti-human Cytokeratin 7 (CK7) | R17-S | DB Biotech®, Slovak Republic | 1:100 | Cytoplasm | Lab Vision® UltraVision® Large Volume Detection System, Thermo Fisher Scientific®, USA |
Monoclonal mouse anti-human Keratin 20 (CK20) | Ks 20.8 | Thermo Fisher Scientific®, USA | 1:100 |