Fig. 4

Quantification of inhibitory effect of MFGM proteins against attachment of F4ac-positive E. coli to the pig enterocytes. Inhibition of F4ac-positive E. coli attachment to porcine enterocytes with various concentrations (5, 25 or 125 μg/ml) of xanthine dehydrogenase (XD), butyrophilin (BTN), lactadherin (LAD), fatty acid binding protein (FAB); positive (rabbit anti-F4ac polyclonal antibody) and negative (rabbit anti-F6 polyclonal antibody or Lactalbumin) controls. Asterisk (*) indicates statistically significant difference between an individual dilution and all of the negative controls (untreated enterocytes + F4ac-positive E. coli, Lactalbumin or anti-F6 antibodies), (P < 0.05). Different letters (a), (b) and (c) indicate statistically significant differences (P < 0.05) between individual dilutions of the same milk fat globule membrane protein. Error bar indicates Standard Deviation. Each column represents mean of quadruplicate measurements in a single representative experiment