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Table 2 Primers used in the study

From: Update on epidemiology of canine babesiosis in Southern France

Gene target PCR target Name Fragment length References
Mitosin gene Canis familiaris CAN-F 5′-CTTGTCACGGTAAGGTTC-3′ 290-bp [31]
CAN-R 5′-CTGATGTATTTCCTGCACCAAG-3′
18S rRNA gene Babesia/Theileria spp. BTF1 (ext) 5′-GGCTCATTACAACAGTTATAG-3′ 930-bp [22]
BTR1 (ext) 5′-CCCAAAGACTTTGATTTCTCTC-3′
BTF2 (int) 5′-CCGTGCTAATTGTAGGGCTAATAC-3′ 800-bp
BTR2 (int) 5′-GGACTACGACGGTATCTGATCG-3′
18S rRNA gene Babesia vogeli BCV-F 5′-GTGTTCGAGTTTGCCATTCG-3′ 422-bp [15]
Ba721R 5′-CCCCAGAACCCAAAGACTTTGATTTCTCTCAAG-3′ [32]
Mitochondrial 16S rRNA gene Ixodida TQ16S + 1 F 5′-CTGCTCAATGATTTTTTAAATTGCTGTGG-3′ 320-bp [33]
TQ16S-2R 5′-ACGCTGTTATCCCTAGAG-3′
Mitochondrial 12S rRNA gene Ticks Forward 5′-AAACTAGGATTAGATACCCTATTATTTTAG-3′ 400-bp [17]
Reverse 5′-CTATGTAACGACTTATCTTAATAAAGAGTG-3′