Update on epidemiology of canine babesiosis in Southern France

BMC Veterinary Research

Table 2 Primers used in the study

Gene target	PCR target	Name		Fragment length	References
Mitosin gene	Canis familiaris	CAN-F	5′-CTTGTCACGGTAAGGTTC-3′	290-bp	[31]
Mitosin gene	Canis familiaris	CAN-R	5′-CTGATGTATTTCCTGCACCAAG-3′	290-bp	[31]
18S rRNA gene	Babesia/Theileria spp.	BTF1 (ext)	5′-GGCTCATTACAACAGTTATAG-3′	930-bp	[22]
		BTR1 (ext)	5′-CCCAAAGACTTTGATTTCTCTC-3′	930-bp
		BTF2 (int)	5′-CCGTGCTAATTGTAGGGCTAATAC-3′	800-bp
		BTR2 (int)	5′-GGACTACGACGGTATCTGATCG-3′	800-bp
18S rRNA gene	Babesia vogeli	BCV-F	5′-GTGTTCGAGTTTGCCATTCG-3′	422-bp	[15]
18S rRNA gene	Babesia vogeli	Ba721R	5′-CCCCAGAACCCAAAGACTTTGATTTCTCTCAAG-3′	422-bp	[32]
Mitochondrial 16S rRNA gene	Ixodida	TQ16S + 1 F	5′-CTGCTCAATGATTTTTTAAATTGCTGTGG-3′	320-bp	[33]
Mitochondrial 16S rRNA gene	Ixodida	TQ16S-2R	5′-ACGCTGTTATCCCTAGAG-3′	320-bp	[33]
Mitochondrial 12S rRNA gene	Ticks	Forward	5′-AAACTAGGATTAGATACCCTATTATTTTAG-3′	400-bp	[17]
Mitochondrial 12S rRNA gene	Ticks	Reverse	5′-CTATGTAACGACTTATCTTAATAAAGAGTG-3′	400-bp	[17]

ISSN: 1746-6148