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Fig. 2 | BMC Veterinary Research

Fig. 2

From: A novel antigen capture ELISA for the specific detection of IgG antibodies to elephant endotheliotropic herpes virus

Fig. 2

Western blot analysis of an irrelevant 27 kD his-tagged protein and (lane 1), single NiNTA purified EEHV-gBHis (lane 2) and double purified NiNTA/HisTrap FPLC (lane 3) with three different antisera. a Western blot with mouse monoclonal anti-His demonstrates the 27 kD His-tagged protein in lane 1 and the highly fragmented EEHV-gBHis in lane 2 and 3. Double purification results in loss of the full length EEHV-gBHis (lane 3). bThe rabbit peptide (ANVTSRRRKRDANTA) EEHV-gB specific antiserum recognizes non-his tagged bands. c Serum of elephant 4A detects identical bands as the rabbit peptide specific serum in the EEHV-gBHis recombinant protein, except for the ~32 kD band which might be an E.coli background band (panel b)

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BMC Veterinary Research

ISSN: 1746-6148

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