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Fig. 1 | BMC Veterinary Research

Fig. 1

From: A novel antigen capture ELISA for the specific detection of IgG antibodies to elephant endotheliotropic herpes virus

Fig. 1

SDS-PAGE total protein analysis (panel a) and Western blot analysis with an anti-His monoclonal antibody (panel b) demonstrate the purification of full length EEHV-gBHis (~87 kD) and several smaller fragments with a his-tag produced from the EEHV-gBHis open reading frame (lane EL, eluate). During purification a small portion of the recombinant protein is lost in the flow through of the NiNTA column (lane FT) and during the washing steps (lane W1-2, W3-4, W5-6). The empty vector does not yield any his-tagged proteins (lane UT). Lane M is the molecular weight marker

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BMC Veterinary Research

ISSN: 1746-6148

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