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Fig. 4 | BMC Veterinary Research

Fig. 4

From: Inhibition of porcine reproductive and respiratory syndrome virus replication in vitro using DNA-based short antisense oligonucleotides

Fig. 4

Viral ORF7 RNA level was reduced by transfection with selected antisense oligonucleotides in both Marc-145 and PAM cells. Four of the nine selected antisense oligonucleotides (5UP2*, YN8, YN4 and YN5) significantly inhibited the RNA level of gene ORF7. After virus challenge and transfection with AS-ONs under the same conditions as in the CPE analysis experiment, total RNA was isolated and subjected to reverse transcription and qPCR analysis. The ΔΔCt method for relative quantification of gene expression was used to determine viral RNA levels. The Y-axis shows the relative RNA levels of the ORF7 gene for each treatment after normalization to the non-transfected reference sample using β-actin as internal control. The values shown are means of three independent experiments

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