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Table 5 Recommended IDT concentrations for most allergen suppliers

From: Canine atopic dermatitis: detailed guidelines for diagnosis and allergen identification

Allergen

Recommended allergen dilution for IDT [49]

Revised recommended allergena dilution for IDT [51, 52, 60]

Histamine

1: 100,000 w/v

1:10,000 w/v

Pollens and moulds

1,000 PNU/mL

1000 to 8000 PNU/mL

Individual DM

250 PNU/mL or 1:50,000 w/v

100–200 PNU/mL (D. pteronyssinus)

75 PNU/mL (D. farina, Tyrophagus putrescentiae, and Lepidoglyphus destructor)

50 PNU/mL (Acarus siro and Blomia tropicalis)

Epidermal extracts

250–500 PNU/mL

At least 1,250 PNU/mL

300 PNU/mL (human dander)

Insects

1,000 PNU/mL

At least 1,750 PNU/mL

Whole flea extract

1:1,000 w/v

1:500 w/v

  1. PNU Protein Nitrogen Units, w/v weight to volume, DM dust mites, D Dermatophagoides, Epidermal extracts: hair, wool, feathers, and dander
  2. aAllergens from Greer Laboratories Inc., Lenoir, NC, USA