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Table 1 Reproducibility of a fluorescence-based real-time polymerase chain reaction for porcine circovirus-like virus P1

From: Development of real-time PCR assay for detection of porcine circovirus-like virus P1 in domestic pigs in China

Copy number

Threshold cycle (Replicates of same batch of samples)

Coefficient of variation (%)

Threshold cycle (Replicates of different batches)

Coefficient of variation (%)

107

12.48

12.51

12.50

0.12

12.50

13.26

12.74

3.04

106

15.57

15.67

15.46

0.67

15.57

16.11

15.55

2.02

105

19.10

18.98

18.94

0.44

19.01

20.10

19.11

3.12

104

22.73

22.90

22.83

0.37

22.82

23.73

22.53

2.72