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Table 1 Reproducibility of intra- and inter-assay with different dilution standard plasmids by SYBR Green real-time RT-PCR

From: Development and evaluation of a SYBR Green real-time RT-PCR assay for detection of avian hepatitis E virus

Variation Different dilutions Ct values for the different dilution of standard plasmids
1 2 3 Mean Ct ± SD CV (%)
Inter-assay 10−5 27.91 28.23 28.07 28.07 ± 0.16 0.58
10−4 23.24 25.67 25.38 24.76 ± 1.33 5.37
10−3 21.89 21.97 22.03 21.96 ± 0.07 0.32
10−2 17.25 18.12 18.23 17.87 ± 0.54 3.02
10−1 14.10 14.02 14.97 14.36 ± 0.53 3.69
Intra-assay 10−5 27.38 27.54 28.01 27.64 ± 0.33 1.19
10−4 25.10 25.23 24.98 25.10 ± 0.13 0.52
10−3 22.07 22.16 22.13 22.12 ± 0.05 0.23
10−2 17.98 18.12 18.05 18.05 ± 0.07 0.39
10−1 14.54 14.78 15.01 14.78 ± 0.24 1.62