Development and evaluation of a SYBR Green real-time RT-PCR assay for detection of avian hepatitis E virus

BMC Veterinary Research

Table 1 Reproducibility of intra- and inter-assay with different dilution standard plasmids by SYBR Green real-time RT-PCR

Variation	Different dilutions	Ct values for the different dilution of standard plasmids
Variation	Different dilutions	1	2	3	Mean Ct ± SD	CV (%)
Inter-assay	10⁻⁵	27.91	28.23	28.07	28.07 ± 0.16	0.58
	10⁻⁴	23.24	25.67	25.38	24.76 ± 1.33	5.37
	10⁻³	21.89	21.97	22.03	21.96 ± 0.07	0.32
	10⁻²	17.25	18.12	18.23	17.87 ± 0.54	3.02
	10⁻¹	14.10	14.02	14.97	14.36 ± 0.53	3.69
Intra-assay	10⁻⁵	27.38	27.54	28.01	27.64 ± 0.33	1.19
	10⁻⁴	25.10	25.23	24.98	25.10 ± 0.13	0.52
	10⁻³	22.07	22.16	22.13	22.12 ± 0.05	0.23
	10⁻²	17.98	18.12	18.05	18.05 ± 0.07	0.39
	10⁻¹	14.54	14.78	15.01	14.78 ± 0.24	1.62

ISSN: 1746-6148