Table 1 Primers for RT-qPCR measurement of antimicrobial peptide gene expression
Gene | Forward Primer (5´-3´) | Reverse Primer (5´-3´) | Amplicon size (bp) | Efficiency |
|---|---|---|---|---|
EF1-α (Reference gene) | CACACTGCTCACATTGCTTGC | ACAATGGCAGCATCTCCAGAC | 209 | 2.07 |
RPL8 (Reference gene) | CACAGAAAGGGGCTGCTAAG | CAGGATGGGTTTGTCAATACG | 254 | 2.09 |
Brevinin-1P (Target gene) | TGAAACGGATGTTGAAGTGG | GTGATTGCCATCTGGTGTGC | 178 | 2.09 |
Ranatuerin-2P (Target gene) | CCAAAGATGTTCACCATGA | CATATGTCCGGCCAAATTCT | 187 | 1.99 |