Immune efficacy of five novel recombinant Bordetella bronchiseptica proteins

BMC Veterinary Research

Table 2 Splenocyte proliferation and cytokine analysis of immune-protective proteins

Group (n = 3)	Cytokine concentration (pg/ml)^a				SI^b
Group (n = 3)	IFN- γ	IL-2	IL-4	IL-10	SI^b
rPPP	625.92 ± 5.91*	18.54 ± 1.41*	202.11 ± 7.37*	320.67 ± 8.96	1.47 ± 0.11
rPL	676.07 ± 5.52*	17.91 ± 0.28*	233.19 ± 13.10*	452.35 ± 12.91*	1.59 ± 0.13
Negative control	546.97 ± 9.39	14.76 ± 0.97	121.81 ± 6.60	288.66 ± 3.60	0.81 ± 0.08

Splenocytes were harvested from the mice 2 weeks after the second immunization with rPPP or rPL. Data represent the mean ± SD from three independent experiments; n = 3 mice per group. Single-cell suspensions of splenocytes were stimulated with an optimized concentration of corresponding recombinant protein
*P < 0.05 compared to the control groups
^aValues for IL-2, IL-4, IL-10, and IFN-γ are titers at 60 h
^bSI stands for stimulation index

ISSN: 1746-6148