Table 1 The primer sequences used to amplify the selected proteins
From: Immune efficacy of five novel recombinant Bordetella bronchiseptica proteins
Primer name | Sequence (5′-3′) | Target gene (reference Gene ID) | PCR amplicon size |
|---|---|---|---|
ABC-F | GCGCCATGGGCAAGAAAATCACCGCTGTA | ABC (ID:2660093) | 789 bp |
ABC-R | TATCTCGAGCTTGATGATGTTGGCGCCGAA | ||
BPP-F | GCGCCATGGGCAACAAGGCATTTCGTTTC | BPP (ID: 2663902) | 1125 bp |
BPP-R | GCGCTCGAGTTACTTGTACGACGTCTTGCT | ||
CHP-F | TATCCATGGGCTATCCCAACCGCCGGCTGTAC | CHP (ID: 2660968) | 573 bp |
CHP-R | TATCTCGAGCTACTTGCCGCGCCCGCCTTG | ||
PL-F | GCGCCATGGGCCGTATGAACAAACGTCAT | PL (ID: 2662792) | 1125 bp |
PL-R | GCGCTCGAGTCAGACCATCTTGTCCTTCAG | ||
PPP-F | GCGCCATGGGCAAAAAGACTCTGCTCGCT | PPP (ID: 2660513) | 1164 bp |
PPP-R | GCGCTCGAGTTAGAAGCGGTGACGGATACC |