Fig. 2
From: Immune efficacy of five novel recombinant Bordetella bronchiseptica proteins
Expression and western blot analysis of recombinant target proteins. Lanes are as follows: Lane M, protein molecular weight marker. Lanes 1–2, 4–5, 7–8, 10–11, 13–14: CBB staining of the recombinant E. coli whole-cell lysate before and after induction with IPTG for recombinant ABC, BPP, PPP, PL, and CHP, respectively. Lanes 3, 6, 9, 12, 15: purified recombinant ABC, BPP, PPP, PL, and CHP, respectively. Lanes 16–17, 18–19, 20–21, 22–23, 24–25: western blot analysis of the purified recombinant ABC, BPP, PPP, PL, and CHP with convalescent sera of rabbits before and after infection with B. bronchiseptica HB, respectively