Dog1
| Date of sampling | CPV2,3
|
Babesia spp.
2
|
Ehrlichia canis
2
|
Leishmania infantum
2
|
Dirofilaria immitis
2
|
|---|
Dog 1
| 28.10.13 | neg | neg 5
| neg 8
| neg 10
| neg 12
|
Dog 2
| 05.11.13 | neg | neg 5
| neg 8
| neg 10
| neg 12
|
Dog 3
| 25.10.13 | Â |
pos
4
| Â | Â | Â |
| Â | 06.11.13 | neg |
pos
5
| neg 8
| neg 10
| neg 12
|
Dog 4
| 22.10.13 | Â | neg 4
| neg 9
|
pos
11
| Â |
Dog 5
| 5.11.13 | neg | neg 5
| neg 8
| neg 10
| neg 12
|
Dog 6
| 5.11.13 | neg | neg 5
| neg 8
| neg 10
| neg 12
|
Dog 7
| 5.11.13 | neg | neg 5
| neg 8
| neg 10
| neg 12
|
Dog 8
| 23.10.13 | Â |
pos
6
| Â | Â | Â |
| Â | 6.11.13 | neg |
pos
5
| neg 8
| interm10
| neg 12
|
Dog 9
| 6.11.13 | neg | neg 5
| neg 8
| neg 10
| neg 12
|
Dog 10
| 7.11.13 | neg | neg 5
| neg 8
| neg 10
| neg 12
|
Dog 11
| 6.11.13 | neg | neg 5
| neg 8
| neg 10
| neg 12
|
Dog 12 | 24.10.13 | Â | interm7
| neg 8
| neg 10
| neg 13
|
| Â | 12.11.13 | neg | Â | Â | Â | Â |
Dog 13 | 12.11.13 | neg | neg 4
| neg 8
| neg 10
|
pos
12,14
|
Total positive (%)
|
0/12 (0Â %)
|
2/13 (15Â %)
|
0/13 (0Â %)
|
1/13 (8Â %)
|
1/13 (8Â %)
|
- Positive results are shown in bold
-
1CDV-positive dogs (at first presentation) are shown in bold; no samples could be collected from Dog 14 (not shown) due to aggressive behaviour; 2
pos positive, neg negative, interm intermediate result, 3Result of CPV real-time qPCR; 4Microscopic evaluation of blood smears for the presence of Babesia spp. organisms by the private veterinarian (Dog 3) or a commercial laboratory (Dog 4: Alomed, Radolfzell-Böhringen, Germany); 5Commercial immunofluorescence antibody test (IFAT) for detecting anti-Babesia canis antibodies (MegaFLUO® BABESIA canis, Megacor Diagnostik GmbH, Hörbranz, Austria) performed by the Institute of Parasitology, University of Zurich; 6Babesia-specific PCR assay from blood performed by a commercial laboratory (Labor am Zugersee, Hünenberg, Switzerland); 7Enzyme-linked immunosorbent assay (ELISA) for the detection of B. canis antibodies performed by a commercial laboratory (IDEXX Diavet AG, Bäch, Switzerland); 8Commercial IFAT for detecting immunoglobulin G antibodies against Ehrlichia canis (Mega Screen Fluoehrlichia canis, Megacor GmbH) performed by the Clinical Laboratory, University of Zurich; 9PCR for detecting E. canis from blood performed by a commercial laboratory (Alomed); 10Published ELISA for the specific detection of anti-Leishmania IgG antibodies [45] performed by the Institute of Parasitology, University of Zurich, using Leishmania infantum promastigote stage antigens and goat anti-dog IgG (γ) antibodies conjugated to alkaline phosphatase (Kirkegaard and Perry Lab, Inc., Maryland, USA); 11IFAT for detecting anti-L. infantum antibodies performed by a commercial laboratory (Alomed); 12commercial Dirofilaria immitis antigen detection test (DiroCHECK, Synbiotics, Lyon, France) performed by the Institute of Parasitology, University of Zurich; 13ELISA for the detection of D. immitis antigen performed by a commercial laboratory (IDEXX Diavet AG); 14Knott Test for the detection of microfilaria of D. immitis performed by the Institute of Parasitology, University of Zurich, as previously published [46]
