Skip to main content

Table 10 Example of a CNS specific processing/embedding cycle [45]

From: International veterinary epilepsy task force recommendations for systematic sampling and processing of brains from epileptic dogs and cats

Incubation time

Chemical

Temperature

6 h

70 % ethanol

40 °C

4 h

80 % ethanol

40 °C

4 h

90 % ethanol

40 °C

4 h

100 % ethanol

40 °C

4 h

100 % ethanol

40 °C

4 h

100 % ethanol

40 °C

2 h

xylene

40 °C

2 h

xylene

40 °C

2 h

xylene

40 °C

1 h

paraffina

60 °C

1 h

paraffina

60 °C

1 h

paraffina

60 °C

3 h

paraffina

60 °C

  1. Cycle times differ if fixation is performed with higher concentrations of formalin as occasionally recommended for large specimens
  2. aWe recommend use of paraffin with 4 % DMSO (Paraplast®, Leica Biosystems, Nussloch)
Back to article page