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Table 4 Protocols in immunohistochemistry

From: Immune response of healthy horses to DNA constructs formulated with a cationic lipid transfection reagent

Target Antibody Clone, immuno-globulin subclass Source Catalogue no. Pretreatment for antigen retrieval Dilution final concentration Secondary antibody, dilution Signal amplification Tissue for positive control Isotype control
Cal-protectin Macrophage/MAC 387 MAC 387, mouse IgG1 DCS Immunoline, Hamburg, Germany MI657C01 Proteinase K 5 μg/ml, in PBS, 30 min 1:400 0.1 μg/ml IHC Kit MouseLink, HRP Label (DCS Immunoline) enhancer IHC Kit MouseLink, HRP Label (DCS Immunoline) polymer Tonsil Mouse IgG1 (Dako, Hamburg, Germany)
IL-12 Mouse anti-bovine IL-12 CC301, mouse IgG2a AbD Serotec, Puchheim, Germany MCA1782 None 1:100 10 μg/ml Anti-mouse-biotin (vector laboratories, Burlingame, Canada) 1:200 Vectastain Elite ABC Kit (vector laboratories) Colon Mouse IgG2a (AbD Serotec)
IL-18 Mouse anti-pig IL-18 5-C-5, mouse IgG1 AbD Serotec MCA2094 None 1:1000 1 μg/ml Anti-mouse-biotin (vector laboratories) 1:200 Vectastain Elite ABC Kit (vector laboratories) Colon Mouse IgG1 (Dako)