Skip to main content

Table 4 Protocols in immunohistochemistry

From: Immune response of healthy horses to DNA constructs formulated with a cationic lipid transfection reagent

Target

Antibody

Clone, immuno-globulin subclass

Source

Catalogue no.

Pretreatment for antigen retrieval

Dilution final concentration

Secondary antibody, dilution

Signal amplification

Tissue for positive control

Isotype control

Cal-protectin

Macrophage/MAC 387

MAC 387, mouse IgG1

DCS Immunoline, Hamburg, Germany

MI657C01

Proteinase K 5 μg/ml, in PBS, 30 min

1:400 0.1 μg/ml

IHC Kit MouseLink, HRP Label (DCS Immunoline) enhancer

IHC Kit MouseLink, HRP Label (DCS Immunoline) polymer

Tonsil

Mouse IgG1 (Dako, Hamburg, Germany)

IL-12

Mouse anti-bovine IL-12

CC301, mouse IgG2a

AbD Serotec, Puchheim, Germany

MCA1782

None

1:100 10 μg/ml

Anti-mouse-biotin (vector laboratories, Burlingame, Canada) 1:200

Vectastain Elite ABC Kit (vector laboratories)

Colon

Mouse IgG2a (AbD Serotec)

IL-18

Mouse anti-pig IL-18

5-C-5, mouse IgG1

AbD Serotec

MCA2094

None

1:1000 1 μg/ml

Anti-mouse-biotin (vector laboratories) 1:200

Vectastain Elite ABC Kit (vector laboratories)

Colon

Mouse IgG1 (Dako)