Target | Antibody | Clone, immuno-globulin subclass | Source | Catalogue no. | Pretreatment for antigen retrieval | Dilution final concentration | Secondary antibody, dilution | Signal amplification | Tissue for positive control | Isotype control |
---|---|---|---|---|---|---|---|---|---|---|
Cal-protectin | Macrophage/MAC 387 | MAC 387, mouse IgG1 | DCS Immunoline, Hamburg, Germany | MI657C01 | Proteinase K 5 μg/ml, in PBS, 30 min | 1:400 0.1 μg/ml | IHC Kit MouseLink, HRP Label (DCS Immunoline) enhancer | IHC Kit MouseLink, HRP Label (DCS Immunoline) polymer | Tonsil | Mouse IgG1 (Dako, Hamburg, Germany) |
IL-12 | Mouse anti-bovine IL-12 | CC301, mouse IgG2a | AbD Serotec, Puchheim, Germany | MCA1782 | None | 1:100 10 μg/ml | Anti-mouse-biotin (vector laboratories, Burlingame, Canada) 1:200 | Vectastain Elite ABC Kit (vector laboratories) | Colon | Mouse IgG2a (AbD Serotec) |
IL-18 | Mouse anti-pig IL-18 | 5-C-5, mouse IgG1 | AbD Serotec | MCA2094 | None | 1:1000 1 μg/ml | Anti-mouse-biotin (vector laboratories) 1:200 | Vectastain Elite ABC Kit (vector laboratories) | Colon | Mouse IgG1 (Dako) |