mRNA expression of genes involved in inflammation and haemostasis in equine fibroblast-like synoviocytes following exposure to lipopolysaccharide, fibrinogen and thrombin

Table 1 Equine specific primers used for quantitative real-time reverse transcriptase PCR

Primer	Primer sequence (5′ → 3′)	Product	NCBI source/
name	Forward/reverse	size	Reference
SAA	CCT GGG CTG CTA AAG TCA TC/	169 bp	AF240364.1
	AGG CCA TGA GGT CTG AAG TG
IL-6	ATG GCA GAA AAA GAC GGA TG/	220 bp	[72]
	GGG TCA GGG GTG GTT ACT TC
MCP-1	ATT GGC CAA GGA GAT CTG TG/	166 bp	EU779497.1
	ATA TCA GGG GGC ATT TAG GG
TF	TGC ACT AGC CAA CAC AAA GC/	101 bp	XM_001491449.2
	CAG AGA CAC AGC CAG GAT GA
uPA	ATG TAT GGT GAT GCC CGT TT/	175 bp	XM_001502951.3
	CAC AGC ATT TTG GTG GTG AC
PAI-1	AAG GGT CCG CTT CCT ACA AT/	204 bp	XM_001492517.3
	TTG AAC TGC ATT GCC TCT TG
VEGF	CAA CGA CGA GGG CCT AGA GT/	100 bp	[73]
	CAT CTC TCC TAT GTG TGG CTT TG
PAR1	TTC GTG ATA AGC CTG CCT CT/	212 bp	XM_001503957.2
	GTA AAA CGC TGC AGT GAC GA
GAPDH	GGG TGG AGC CAA AAG GGT CAT CAT/	417 bp	[74]
	AGC TTT CTC CAG GCG GCA GGT CAG

Primers used to amplify specific genes in LPS, fibrinogen, thrombin and non-treated equine fibroblast-like synoviocytes: SAA: serum amyloid A. IL-6: interleukin-6. MCP-1: monocyte chemotactic protein-1. TF: tissue factor. uPA: urokinase plasminogen activator. PAI-1: plasminogen activator inhibitor-1. VEGF: vascular endothelial growth factor. PAR-1: protease activator receptor-1. GAPDH: gluceraldehyde‐3‐phosphate dehydrogenase

ISSN: 1746-6148