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Table 1 Equine specific primers used for quantitative real-time reverse transcriptase PCR

From: mRNA expression of genes involved in inflammation and haemostasis in equine fibroblast-like synoviocytes following exposure to lipopolysaccharide, fibrinogen and thrombin

Primer Primer sequence (5′ → 3′) Product NCBI source/
name Forward/reverse size Reference
SAA CCT GGG CTG CTA AAG TCA TC/ 169 bp AF240364.1
  AGG CCA TGA GGT CTG AAG TG   
IL-6 ATG GCA GAA AAA GAC GGA TG/ 220 bp [72]
  GGG TCA GGG GTG GTT ACT TC   
MCP-1 ATT GGC CAA GGA GAT CTG TG/ 166 bp EU779497.1
  ATA TCA GGG GGC ATT TAG GG   
TF TGC ACT AGC CAA CAC AAA GC/ 101 bp XM_001491449.2
  CAG AGA CAC AGC CAG GAT GA   
uPA ATG TAT GGT GAT GCC CGT TT/ 175 bp XM_001502951.3
  CAC AGC ATT TTG GTG GTG AC   
PAI-1 AAG GGT CCG CTT CCT ACA AT/ 204 bp XM_001492517.3
  TTG AAC TGC ATT GCC TCT TG   
VEGF CAA CGA CGA GGG CCT AGA GT/ 100 bp [73]
  CAT CTC TCC TAT GTG TGG CTT TG   
PAR1 TTC GTG ATA AGC CTG CCT CT/ 212 bp XM_001503957.2
  GTA AAA CGC TGC AGT GAC GA   
GAPDH GGG TGG AGC CAA AAG GGT CAT CAT/ 417 bp [74]
  AGC TTT CTC CAG GCG GCA GGT CAG   
  1. Primers used to amplify specific genes in LPS, fibrinogen, thrombin and non-treated equine fibroblast-like synoviocytes: SAA: serum amyloid A. IL-6: interleukin-6. MCP-1: monocyte chemotactic protein-1. TF: tissue factor. uPA: urokinase plasminogen activator. PAI-1: plasminogen activator inhibitor-1. VEGF: vascular endothelial growth factor. PAR-1: protease activator receptor-1. GAPDH: gluceraldehyde‐3‐phosphate dehydrogenase