Table 1 Primers used in the present study
Name | Sequence | Application |
|---|---|---|
3β-HSD (NM_001004049.1) | F: TTCCTGGCAAGTATTTCTCGG | mRNA expression |
R: TCCAGCAACAAGTGGACGAT | ||
GR (AY779185) | F: CCAAACTCTGCCTTGTGTGTTC | |
R: TGTGCTGTCCTTCCACTGCT | ||
C/EBPβ (NM_001199889.1) | F: GACAAGCACAGCGACGAGTA | |
R: AGCTGCTCCACCTTCTTCTG | ||
AR (NM_214314.2) | F: AGCACCATACGGCTACAC | |
R: GCCCATCTCGCTTTTGAC | ||
PPIA (NM_214353.1) | F: GACTGAGTGGTTGGATGG | |
R: TGATCTTCTTGCTGGTCTT | ||
C/EBPβ-Site1 (−2078/-1953) | F: ACTGCGGAGGGGCTATTG | ChIP for 3β-HSD promoter AR, GR and C/EBPβ binding |
R: CTCAGAACTCATGCGACCAG | ||
C/EBPβ-Site2/3 GR (−723/-576) | F: TGAGACTTTGGCCGCAATC | |
R: GTGACTTGTGGCCTTATGCA | ||
C/EBPβ-Site4 GR (−45/+171) AR (−45/+171) | F: GCTGAGTCAGAGGCAAGGG | |
R: GAGGTCAGGTGATTCAGTGGAA | ||
GR (−371/-178) | F: TAATCCACTGGGCGAGAC | |
R: AACCACAGCAGGAGAAAGAT | ||
AR (−298/-79) | F: CCATGACAGGGACTCCTCC | |
R: AGCTCACGCCTCCTTACTTC | ||
AR (−5279/-4985) | F: CCTCAAGGATAGAAGGATGG | |
R: CAATGGGGAAAAGCGTAG |