A porcine reproductive and respiratory syndrome virus (PRRSV) vaccine candidate based on the fusion protein of PRRSV glycoprotein 5 and the Toll-like Receptor-5 agonist Salmonella Typhimurium FljB

Table 1 PCR primers used in this study

Primer name	Primer sequence (5′ → 3′)	Application
GP5-ORF F	ACTTAAGCTTGGTACCATGG	Amplification of GP5 ORF
GP5-ORF R	CGCTAGAGCGCTGGCAAGTG	Amplification of GP5 ORF
GP5a F	CCGGGATCCAACGCCAGCGACAACAAC	Amplification of GP5a fragment
GP5a R	CACCGCCGCTTCCACCGCCACCCTCCACTGCCCAGTCAAA	Amplification of GP5a fragment
GP5b F	GTGGAAGCGGCGGTGGCGGAAGCTGCATGTCCTGGCGCTA	Amplification of GP5b fragment
GP5b R	CCGGAATTCGAGACGACCCCATAGTTCCGCT	Amplification of GP5b fragment
fljB F	GGGGGAATTC GGTGGCGGTGGTTCTATGGCACAAGTAATCAACACTAACAGT	Amplification of fljB fragment
fljB R	GCGTCGACTTAACGTAACAGAGACAGC	Amplification of fljB fragment

ISSN: 1746-6148