Fig. 7

PRRSV-specific IgG antibody titers in serum at day 12 after the third immunization. C3H/HeJ mice were randomly divided into five groups (6 mice per group) and immunized intraperitoneally either with rGP5, rGP5-FljB, rGP5 + R848, rGP5 + aluminium adjuvant, or PBS, respectively. These mice were immunized three times on days 0, 14, and 28 at a dose of 50 μg rGP5, 50 μg rGP5-FljB, 10 μg R848 or isochoric aluminium adjuvant in 200 μL. Blood was collected from eye sockets on days 40 for analysis of anti-PRRSV IgG titers by ELISA. Data reflects the mean ± SD by using the Student t test at p < 0.01 (**) or p < 0.001 (***)