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Fig. 6 | BMC Veterinary Research

Fig. 6

From: A porcine reproductive and respiratory syndrome virus (PRRSV) vaccine candidate based on the fusion protein of PRRSV glycoprotein 5 and the Toll-like Receptor-5 agonist Salmonella Typhimurium FljB

Fig. 6

Immunization schedule and GP5-specific IgG antibody titers in serum. (a) C3H/HeJ mice were randomly divided into five groups (6 mice per group) and immunized intraperitoneally either with rGP5, rGP5-FljB, rGP5 + R848, rGP5 + aluminium adjuvant, or PBS, respectively. These mice were immunized three times on days 0, 14, and 28 at a dose of 50 μg rGP5, 50 μg rGP5-FljB, 10 μg R848 or isochoric aluminium adjuvant in 200 μL. Blood was collected from eye sockets on days 26, 40, 52 and 64 for analysis of anti-GP5 IgG titers by ELISA. (b) GP5-specific IgG antibody titers in serum at day 12 after the boost and third immunization. (c) IgG1 and IgG2a levels in serum at day 12 after the third immunization. Data reflects the mean ± SD by using the Student t test at p < 0.05 (*), p < 0.01 (**) or p < 0.001 (***)

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