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Fig. 4 | BMC Veterinary Research

Fig. 4

From: A porcine reproductive and respiratory syndrome virus (PRRSV) vaccine candidate based on the fusion protein of PRRSV glycoprotein 5 and the Toll-like Receptor-5 agonist Salmonella Typhimurium FljB

Fig. 4

Western blotting analysis of His-rGP5 and rGP5-FljB proteins. (a) Analysis of His-rGP5 and rGP5-FljB with an anti-PRRSV polyclonal antibody. Lanes: M, molecular weight markers; 1, 1 × SDS-loading buffer; 2, purified His-rGP5 protein; 3, purified rGP5-FljB protein. (b) Analysis of rGP5-FljB with an anti-FljB polyclonal antibody. Lanes: M, molecular weight markers; 1, Lysate supernatant of BL21(DE3)(pCold-rGP5-FljB) induced by IPTG; 2, Inclusion bodies of BL21(DE3)(pCold-rGP5-FljB) induced by IPTG; 3, product of BL21(DE3)(pCold) induced by IPTG

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