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Fig. 1 | BMC Veterinary Research

Fig. 1

From: A porcine reproductive and respiratory syndrome virus (PRRSV) vaccine candidate based on the fusion protein of PRRSV glycoprotein 5 and the Toll-like Receptor-5 agonist Salmonella Typhimurium FljB

Fig. 1

Schematic representation for the construction of rGP5 and rGP5-fljB. rGP5 fragment with the deletion of its signal peptide (green) and transmembrane regions (light grey) was amplified by overlap-PCR, and inserted into the BamHI and EcoRI digested expression vector pColdI or pGEX-6p-1 to create pCold-rGP5 or pGEX-6p-1-rGP5 respectively. Flagellin fljB gene (light blue) was amplified from the genomic DNA of attenuated Salmonella Typhimurium SL7207 strain and cloned into the EcoRI and SalI sites of pCold-rGP5, resulting in a recombinant plasmid pCold-rGP5-fljB. “L” represents the linker sequence GGGGS

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