Development of an improved competitive ELISA based on a monoclonal antibody against lipopolysaccharide for the detection of bovine brucellosis

Table 1 Indirect ELISA results for hybridoma supernatants

MAb	Ig class	OD^a										Epitope recognized
MAb	Ig class	Y. enterocolitica O:9	E. coli O157	S. gallinarum	O. anthropi	F. tularensis	P. multocida	RM6/66(R)	NI (A⁺M⁺)	16 M (A⁻M⁺)	2308 (A⁺M⁻)	Epitope recognized
2C3	IgG3	0.043	0.038	0.027	0.035	0.030	0.033	0.044	1.176	0.856	0.040	M
3E3	IgG1	0.037	0.053	0.036	0.039	0.031	0.042	0.041	1.419	0.942	0.044	M
6E3	IgG1	0.040	0.026	0.023	0.028	0.025	0.024	0.031	1.086	0.907	0.060	M
3H7	IgG3	0.350	0.061	0.046	0.045	0.043	0.057	0.035	1.115	0.567	1.544	C/Y(A > M)
3 F9	IgG3	0.037	0.053	0.031	0.042	0.037	0.049	0.034	1.022	0.983	0.514	C(M > A)
4C3	IgG2a	0.422	0.031	0.028	0.033	0.027	0.030	0.043	0.647	0.687	0.605	C/Y(M = A)
II5G1	IgG1	0.434	0.063	0.038	0.048	0.041	0.045	0.034	0.906	0.882	1.113	C/Y(M = A)
II4D11	IgG3	0.557	0.043	0.034	0.041	0.031	0.042	0.032	0.938	0.930	0.899	C/Y(M = A)
6B8	IgM	1.039	0.037	0.030	0.036	0.032	0.039	0.205	0.837	0.913	0.991	C/Y(M = A)
6 F2	IgM	0.959	0.023	0.021	0.033	0.031	0.024	0.048	0.897	0.899	0.845	C/Y(M = A)
4H7	IgM	0.442	0.030	0.028	0.035	0.030	0.034	0.043	0.946	0.853	0.898	C/Y(M = A)
I2C10	IgM	0.503	0.058	0.033	0.040	0.028	0.044	0.040	0.896	0.689	1.317	C/Y(A > M)

^aOD of hybridoma supernatants in dilutions of OD 1.0 in iELISA with LPS of B.melitensis 16 M

ISSN: 1746-6148