Skip to main content
Fig. 4 | BMC Veterinary Research

Fig. 4

From: Development of an improved competitive ELISA based on a monoclonal antibody against lipopolysaccharide for the detection of bovine brucellosis

Fig. 4

Establishment of the cELISA. (A) Percent inhibition values of 63 negative serum samples by cELISA. A cut-off value was set at 40 % (mean + 2SD). (B) Percent inhibition of polyclonal antisera against various bacteria serological related with smooth Brucella. 1 Y. enterocolitica O:9; 2 E. coli O157; 3 S. gallinarum; 4 O. anthropic ATCC49188; 5 F. tularensis LVS; 6 P. multocida; 7 positive bovine sera. Only the positive bovine sera had a PI exceeding the cut-off value. The Error bars indicate the standard deviations from three-well replications for each serum sample

Back to article page