Fig. 3From: Development of an improved competitive ELISA based on a monoclonal antibody against lipopolysaccharide for the detection of bovine brucellosisWestern blot analysis for epitope of MAbs. Whole-cell extracts of B. canis RM6/66 (native rough type), B. melitensis NI (smooth type, A+M+), B. melitensis 16 M (smooth type, M-dominant, A−M+), B. abortus 2308 (smooth type, A-dominant, A+M−) were used to analyze the epitopic specificity of MAbs. SDS-PAGE and Western blot were carried out as described in Materials and Methods Back to article page