Skip to main content

Table 4 Results of RT-qPCR to evaluate in vitro expression of transgenes from MIDGE-Th1 vectors encoding eqIL12, eqIL18, htyr and hgp100

From: Local and systemic effect of transfection-reagent formulated DNA vectors on equine melanoma

MIDGE-Th1 Estimated mRNA / cDNA copy numbers per transgene (Ct values)
vectors [μg] eqIL12-p35 eqIL12-p40 eqIL18 htyr hgp100
eqIL12, eqIL18 1.14 + E03 1.52 + E04 3.16 + E03 < LOD < LOD
[0.5 + 0.5] (27) (23) (25)
eqIL12, eqIL18, htyr 1.01 + E03 2.10 + E04 5.23 + E03 3.01 + E04 < LOD
[0.5 + 0.5 + 1.25] (28) (23) (25) (22)
eqIL12, eqIL18, 9.10 + E02 9.79 + E03 1.98 + E03 < LOD 1.30 + E04
gp100 (28) (24) (26)   (23)
[0.5 + 0.5 + 1.25]
  1. LOD (limit of detection): = < 25 copies/reaction.