Reactive oxygen species generation by bovine blood neutrophils with different CXCR1 (IL8RA) genotype following Interleukin-8 incubation

Table 1 Statistical analysis of reactive oxygen species generation by blood neutrophils following phorbol 12-myristate 13-acetate stimulation

Polymorphism ¹	Fixed effect	n ²	AUC ³			RLUmax ⁴			Tmax ⁵
Polymorphism ¹	Fixed effect	n ²	β ⁶	SE ⁷	P ⁸	β ⁶	SE ⁷	P ⁸	β ⁶	SE ⁷	P ⁸
c.735C > G	Intercept		71	8		37	6		9.2	1.0
	Genotype				0.69			0.94			0.47
	c.735CC	7	Ref.⁹	…		Ref.⁹	…		Ref.⁹	…
	c.735CG	6	-6	11		-3	9		0.3	1.3
	c.735GG	7	-9	11		-1	8		-1.2	1.3
	Incubation				<0.01			<0.01			<0.05
	2 h		Ref.⁹	…		Ref.⁹	…		Ref.⁹	…
	6 h		11	2		4	1		1.2	0.5
	rbIL-8				<0.01			0.17			<0.01
	0 ng/ml		Ref.⁹	…		Ref.⁹	…		Ref.⁹	…
	40 ng/ml		7	2		-3	2		3.1	0.6
	400 ng/ml		5	2		-2	2		3.1	0.6
c.980A > G	Intercept		66	11		29	9		8.9	1.5
	Genotype				0.45			0.56			0.78
	c.980AA	3	Ref.⁹	…		Ref.⁹	…		Ref.⁹	…
	c.980AG	5	10	14		12	11		0.7	1.8
	c.980GG	12	-4	13		5	10		-0.2	1.6
	Incubation				<0.01			<0.01			<0.05
	2 h		Ref.⁹	…		Ref.⁹	…		Ref.⁹	…
	6 h		11	2		4	1		1.2	0.5
	rbIL-8				<0.01			0.17			<0.01
	0 ng/ml		Ref.⁹	…		Ref.⁹	…		Ref.⁹	…
	40 ng/ml		7	2		-3	2		3.1	0.6
	400 ng/ml		5	2		-2	2		3.1	0.6

¹Linear mixed regression models describing the association between reactive oxygen species generation by blood neutrophils and CXCR1 polymorphisms c.735C > G and c.980A > G, respectively. Neutrophils were incubated with 0, 40 or 400 ng recombinant bovine interleukin 8 per mL for 2 or 6 h and stimulated with phorbol 12-myristate 13-acetate,
²Number of heifers,
³Area under the curve values in 10⁶ RLU (relative light units) * s,
⁴Peak values in 10³ RLU.
⁵Time of peak values in min,
⁶Regression coefficient,
⁷Standard error,
⁸Overall P-value of the fixed effect,
⁹Reference,
All two-way interactions between the fixed effects were non-significant (P > 0.05) and removed from the model.

ISSN: 1746-6148