Figure 3

ARV S1133 activates RhoA and ROCK1.Vero cells infected with ARV S1133 at an MOI of 5 for different time periods. (A) Cell lysates reacted with GST-tagged Rhotekin Rho binding domain fusion protein. Active GTP-bound Rho was eluted and subsequent detection was performed by immunoblot analysis using anti-RhoA. Equal amounts of total cell lysates were subjected to SDS-PAGE and the protein levels were detected using the indicated antibodies. (B) 200 μg total cell lysates were added to the plates and incubated, and a detection antibody and substrate were then applied. The amount of phosphorylated substrate was measured at an absorbance of 450 nm. Each experiment was performed three times, each in duplicate. The data are presented as the mean ± SD.