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Table 4 Results of RT-qPCR to evaluate in vitro expression of transgenes from MIDGE-Th1 vectors encoding eqIL12, eqIL18, htyr and hgp100

From: Local and systemic effect of transfection-reagent formulated DNA vectors on equine melanoma

MIDGE-Th1 Estimated mRNA/cDNA copy numbers per transgene (Ct values)
Vectors [μg] eqIL12-p35 eqIL12-p40 eqIL18 htyr hgp100
eqIL12, eqIL18 [0.5 + 0.5] 1.14 + E03(27) 1.52 + E04(23) 3.16 + E03(25) < LOD < LOD
eqIL12, eqIL18, htyr [0.5 + 0.5 + 1.25] 1.01 + E03(28) 2.10 + E04(23) 5.23 + E03(25) 3.01 + E04(22) < LOD
eqIL12, eqIL18, gp100 [0.5 + 0.5 + 1.25] 9.10 + E02(28) 9.79 + E03(24) 1.98 + E03(26) < LOD 1.30 + E04(23)
  1. LOD (limit of detection): = < 25 copies/reaction.