Table 4 Results of RT-qPCR to evaluate in vitro expression of transgenes from MIDGE-Th1 vectors encoding eqIL12, eqIL18, htyr and hgp100
From: Local and systemic effect of transfection-reagent formulated DNA vectors on equine melanoma
MIDGE-Th1 | Estimated mRNA/cDNA copy numbers per transgene (Ct values) | ||||
|---|---|---|---|---|---|
Vectors [μg] | eqIL12-p35 | eqIL12-p40 | eqIL18 | htyr | hgp100 |
eqIL12, eqIL18 [0.5 + 0.5] | 1.14 + E03(27) | 1.52 + E04(23) | 3.16 + E03(25) | < LOD | < LOD |
eqIL12, eqIL18, htyr [0.5 + 0.5 + 1.25] | 1.01 + E03(28) | 2.10 + E04(23) | 5.23 + E03(25) | 3.01 + E04(22) | < LOD |
eqIL12, eqIL18, gp100 [0.5 + 0.5 + 1.25] | 9.10 + E02(28) | 9.79 + E03(24) | 1.98 + E03(26) | < LOD | 1.30 + E04(23) |